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Product Details
| Cat # +Size | K431-40 |
|---|---|
| Size | 40 assays |
| Detection Method | Western blot analysis using a phospho-c-Jun specific antibody at 1:1000 dilutions |
| Species Reactivity | Mammalian |
| Applications | The JNK Activity Immunoassay Kit utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysate. JNK-specific activity is then analyzed by determining the phosphorylation of c-Jun by Western blotting using a phospho-c-Jun specific antibody. |
| Features & Benefits | • Simple procedure • The kit is suitable for both research and drug discovery • The assay utilizes a N-terminal c-Jun fusion protein bead to selectively “pull down” JNK from cell lysate c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody. |
| Kit Components | • Kinase Extraction Buffer • JNK Specific Antibody • Protein A Sepharose • c-Jun Protein/ATP Mixture • Kinase Assay Buffer • Phospho-cJun Specific Antibody |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes. The JNK Activity Immunoassay Kit utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysate. Activity of the JNK is then determined in a kinase reaction using recombinant c-Jun as substrate. Phosphorylation of the c-Jun can be analyzed by Western blot analysis using a phospho-c-Jun specific antibody. The kit specifically detects JNK, other kinase activity would not be detected.
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Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
García-Ruiz et al., High-fat diet decreases activity of the oxidative phosphorylation complexes and causes nonalcoholic steatohepatitis in mice. Dis. Model. Mech., Nov 2014; 7: 1287 - 1296.
Guofa Liu et al., c-Jun N-terminal Kinase 1 (JNK1) Is Required for Coordination of Netrin Signaling in Axon Guidance. J. Biol. Chem., Jan 2013; 288: 1883 - 1895.
Vardarova, K. et al. PKC-alpha and PK Differentially Regulate Legionella pneumophila-Induced GM-CSF. Eur. Respir. J. 2009; 34: 1171-1179.
Yu XX et al (2008) Am J Physiol Endocrinol Metab 295: E436 - E446.
For more citations of this product click here
