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JMJD2A Antibody

Polyclonal antibody to detect JMJD2A (a lysine specific demethylase ) in human samples
Catalog #: 6108

Product Details

Cat # +Size 6108-100
Size 100 μl
Antibody Target JMJD2A
Alternate Name Jumonji Domain Containing 2A; KDM4A; Lysine-Specific Demethylase 4A
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen Human recombinant JMJD2A aa 1-350
Accession # O75164
Gene ID 9682
Appearance Colorless liquid
Formulation 50 µg of antibody in 250 μl TBS, pH 7.4, containing 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide.
Purification Antigen affinity purified
Species Reactivity Human JMJD2A. Expected to cross react with JMJD2C (>90% similarity), JMJD2B (>85% similarity) due to high homology to these JMJD2 paralogs considering the antigen used. Observed detection of purified recombinant human JMJD2D at 50 ng sμggesting only ~10%
Application Western blot
Positive Control Western blot: DLD1 cell lysate
Application & Usage Western blot: 1:200 dilution. Not recommended for FACS and IHC based on probable cross reactivity with other JMJD containing proteins.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Jumonji Domain containing 2A (JMJD2A) is a lysine specific demethylase with emerging roles in histone modification or epigenetic remodeling. This JMJD2A polyclonal antibody was raised against an N-terminal recombinant fragment of JMJD2A. This fragment includes amino acids 1-350 including the JMJN and JMJC domains but not the two LAP/PHD zinc finger or Tudor domains of the 1,064 amino acid protein. JMJD2A is detected at 135 kDa by western blotting using a DLD1 cell lysate as a positive control. Also, the expression of JMJD2A varies by tissue, and northern blotting sμggests skeletal muscle as a negative control and lung as a positive control tissue.

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