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Janus Kinase 1 Inhibitor Screening Kit (Fluorometric)

A 96-well plate based, fluorometric assay for screening potential JAK1 inhibitors.
Catalog #: K2111
$545.00

Product Details

Cat # +Size K2111-100
Size 100 assays
Kit Summary BioVision’s Janus Kinase 1 Inhibitor Screening Kit is a 96-well plate based, fluorometric assay to screen potential JAK1 inhibitors.
Detection Method Fluorescence (Ex/Em = 535/587 nm)
Species Reactivity Human
Applications Screening or characterizing Janus Kinase 1 inhibitors.
Features & Benefits The assay provides a rapid, simple, and reliable test for high-throughput screening of JAK1 inhibitors.
Kit Components ● JAK1 Assay Buffer
● Fluorogenic Probe
● ADP Detection Mix
● Developer Enzyme Mix
● JAK1 Peptide Substrate
● Ultra-Pure ATP
● JAK1 Enzyme
● Filgotinib
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

Janus Kinase 1 (JAK1) is a member of the JAK family of intracellular, non-receptor tyrosine kinase that uses the JAK-STAT pathway in cytokine-mediated signaling. It has two mostly identical, phosphate-transferring domains where one domain exhibits the kinase activity, while the other domain regulates the kinase activity of the first. JAKs play a critical role in regulating diverse signaling pathways that govern cellular survival, proliferation, differentiation and apoptosis. Several JAK inhibitors including baricitinib (Olumiant), tofacitinib (Xeljanz), and upadacitinib (Rinvoq), are approved by the FDA to treat rheumatoid arthritis. Additionally, they are studied for the treatment of atopic dermatitis, essential thrombocythemia, polycythemia vera, psoriasis, alopecia, ulcerative colitis, etc. BioVision’s Janus Kinase 1 Inhibitor Screening Kit is a 96-well plate based, fluorometric assay to screen potential JAK1 inhibitors. The kit uses an active, human JAK1 enzyme, which hydrolyzes the JAK1 substrate and ATP to generate ADP. ADP in the presence of a fluorogenic probe and developing solution yields a fluorescent product, which is measured at Ex/Em = 535/587 nm. However, when JAK1 activity is inhibited by a potent and selective JAK1 inhibitor, filgotinib, the fluorescent signal is reduced. The assay provides a rapid, simple, and reliable test for high-throughput screening of JAK1 inhibitors.


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