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Isopropanol Dehydrogenase Activity Assay Kit (Colorimetric)

Simple and sensitive colorimetric assay for NADP dependant Isopropanol Dehydrogenase (ADH1-NADP) Activity in varied sample types.
Catalog #: K698

Product Details

Cat # +Size K698-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity Mammalian samples. Bacterial, yeast or protozoan samples: Entamoeba histolytica, Clostridium beijerinckii, Mycoplasma pneumonia, etc.
Applications Measurement of Isopropanol Dehydrogenase (ADH1-NADP) activity in various cells.
Measurement of NADP-dependent alcohol dehydrogenases’ activity with broad substrate specificity in various tissues/cells.
Features & Benefits • Simple, colorimetric protocol.
• Detects ADH1-NADP activity as low as 0.001 mU in samples.
• Compatible with multiple sample types.
Kit Components • ADH1-NADP Assay Buffer
• ADH1-NADP Substrate
• ADH1-NADP Developer
• ADH1-NADP Positive Control
• NADPH Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


NADP-dependent Isopropanol Dehydrogenase (ADH1-NADP, EC: belongs to the superfamily of alcohol dehydrogenases (Alcohol DHs, ADHs, EC with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Isopropanol dehydrogenase facilitates the conversion between secondary alcohols to aldehydes and ketones with the reduction of NADP+ to NADPH. BioVision’s Isopropanol Dehydrogenase Activity Colorimetric Assay Kit provides a convenient tool for sensitive detection of the ADH1-NADP in a variety of samples. In this assay, Isopropanol gets converted to Acetone and NADPH in the presence of NADP+. This results in the development of colour in the presence of the developer. This colour is proportional to the ADH1-NADP levels in the sample and can be measured at λ = 450 nm. This assay detects ADH1-NADP activity as low as 0.001 mU in samples.

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Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.