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Isocitrate Dehydrogenase Activity Colorimetric Assay Kit

based on 2 citations in multiple journalsIsocitrate Dehydrogenase Activity Colorimetric Assay Kit24.1 4
Catalog #: K756

In stock

$365.00

Product Details

Cat # +Size K756-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity Mammalian
Applications This assay has a detection sensitivity as low as 0.01 mU.
Features & Benefits • Simple procedure
• Fast and convenient
• Sensitive assays for measuring IDH activity in various biological samples and the kit is also suitable for high throughput analyses.
Kit Components • IDH Assay Buffer
• NADᶧ (lyophilized)
• NADPᶧ (lyophilized)
• IDH Substrate (lyophilized)
• Developer (lyophilized)
• IDH Positive Control (NADPᶧ)
• NADH Standard (0.5 μmol, lyophilized)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In BioVision’s ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ⍺-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well.


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Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Hagras, Abeer M. et al. (2016) Mobile phones electromagnetic radiation and NAD+-dependent isocitrate dehydrogenase as a mitochondrial marker in asthenozoospermia, Biochimie Open. 2016 Dec; 3:19-25
For more citations of this product click here