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IP-10/CXCL10 (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of human IP-10/CXCL10 in plasma, tissue homogenates and other biological fluids.
Catalog #: E4826

Product Details

Cat # +Size E4826-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications A Sandwich ELISA kit for the quantitative measurement of human IP-10/CXCL10 in plasma, tissue homogenates and other biological fluids.
Features & Benefits • Specificity: This kit recognizes Human IP-10/CXCL10 in samples. No Significant cross-reactivity or interference between Human IP-10/CXCL10 and analogues was observed.
• Sensitivity: 4.69 pg/mL
• Variability: Coefficient of variation is < 10%
• Detection Range: 7.81-500 pg/mL
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Chemokine (C-X-C motif) ligand 10 (CXCL10), also known as Interferon gamma-inducible protein-10 (IP-10), is a non-ELR chemokine secreted by various cell types including monocytes, endothelial cells, and fibroblasts in response to IFN-γ. CXCL10 functions via chemokine receptor CXCR3. CXCL10 has been attributed to several roles, such as chemo-attraction for activated T-lymphocytes, inhibition of angiogenesis, and antitumor activity. BioVision’s IP-10/CXCL10 (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IP-10/CXCL10. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IP-10/CXCL10 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IP-10/CXCL10. The concentration of Human IP-10/CXCL10 in the samples can be calculated by comparing the OD of the samples to the standard curve.

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