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Iodide Colorimetric Assay Kit

To determine Iodide concentration
Catalog #: K2037

Product Details

Cat # +Size K2037-100
Size 100 assays
Detection Method Absorbance 330 nm
Species Reactivity All
Applications Determination of Iodide in different samples such as foods, liquid and biological samples
Features & Benefits • Non-radioactive method to determine Iodide concentration in various sample types including foods, liquid and biological samples
• Simple
• Can detect as low as 0.2 μM Iodide under the assay conditions
• Convenient
• Sensitive
Kit Components • Iodide Standard
• Iodide Substrate
• Treatment Reagent
• Sample Diluent
• Precipitation Solution
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Iodide (I−) is a halide anion and a necessary and limiting substrate for thyroid hormone synthesis. This essential element enables the thyroid gland to produce thyroid hormones, thyroxine (T4) and triiodothyronine (T3). Additionally, Iodide is important in many essential biological processes such as basal metabolism, temperature regulation, intellectual development for children, muscular development, normal heart function, growth of skeleton etc. Food is the main source of daily supply of iodide in humans. Iodide can be found in grains, fish, dairy products, fruits, iodized salts etc. Iodine deficiency affects around two billion people worldwide. Iodide deficiency can cause diseases such as goiter, depression, fatigue, hypothyroidism, intellectual disability, delayed sexual development etc. However, excess intake of Iodide is also harmful and can inhibit the release of thyroid hormones and cause inflammation of the thyroid gland and even thyroid cancer. BioVision’s Iodide Colorimetric Assay Kit provides a quick, convenient, non-radioactive method to determine Iodide concentration in various sample types including foods, liquid and biological samples. The assay relies on the Iodide ion as a specific catalyst to convert a yellow colored substrate to a colorless product. The reduction in absorbance signal at 330 nm is directly proportional to the Iodide concentration in the samples. Most common ions (eg. Na+, Ca2+, Cu2+, K+, Mg2+, Fe3+, NH4+, CO32- Br-, Cl-, SO42-, NO3- and PO43-) do not interfere with the assay. The kit is simple, rapid, sensitive and can detect as low as 0.2 μM Iodide under the assay conditions.

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Please recommend the steps to fully dissolve the TBA solution if there is precipiate after bringing up the volume to 25ml with water?
Sonication in a RT water bath can be used if needed. You can also dissolve the TBA in 15 ml of 50% glacial acetic acid in water. Then fill the volume with water to make 25ml. Mix well. This should help in dissolving the TBA. If there is still little precipitate, use 600 ul as directed and any particle in suspension will dissolve at 95C when incubated for 60 mins.
How do you convert μM to PPM for K2037 which has a highest detection limit listed as 5 μM?
1 ppm equals 1 mg/L and Mol. wt of Iodine = 253

Highest concentration K2037 can detect is 5 μM or 0.000005 M

To get g/L = 0.000005 x 253
= 0.001265 or 1.265 mg/L = 1.265 ppm

Therefore, the highest detection limit is 5 μM or 1.265 ppm