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Invertase Activity Colorimetric Assay Kit

Sensitive Assay, HTS
Catalog #: K674

In stock

$385.00

Product Details

Cat # +Size K674-100
Size 100 assays
Detection Method Absorbance (OD 570 nm)
Species Reactivity Bacteria, Mammals
Applications Measurement of Invertase activity in various biological fluids, bacteria and crude enzyme preparations.

Analysis of metabolic pathways such as glycolysis and Diabetes Obesity-related Signaling.
Features & Benefits • Simple, Sensitive & Rapid protocol
• Highly-Sensitive: Detects as low as 20 µU Invertase in various sample types
Kit Components • Invertase Hydrolysis Buffer
• Invertase Assay Buffer
• Invertase Stop Solution
• OxiRed™ Probe (in DMSO)
• Invertase Enzyme Mix
• Invertase Substrate
• Glucose Standard (100 mM)
• Positive Control
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Invertase (EC 3.2.1.26), also known as sucrase or β -fructofuranosidase, catalyzes the hydrolysis of sucrose (table sugar) by cleaving its glycosidic bond and forming one molecule each of glucose and fructose. Invertase is widely expressed among plants and microorganisms including yeast and bacteria. It is extensively utilized in industrial processes, including food, ethanol production, cosmetics, drugs, paper and bioelectronics electrodes. BioVision’s Invertase Activity Assay kit provides a simple, sensitive and high-throughput adaptable assay that detects relevant concentrations of Invertase in biological samples or purified/crude enzyme preparations. The assay is based on the enzymatic detection of glucose, one of the hydrolysis products. Detection limit: 20 µU.


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Can this kit be used to assay honey samples?
We have not tested these kits with honey samples but there is no restriction on using this sample type as long as the sample is diluted to make it aqueous to reduce viscosity and as long as there is active invertase or glucose oxidase in the samples. The sample volume used per assay will need to be optimized such that the values are within the linear range of the std. curve.
Can frozen samples be used with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple times (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Is it possible to use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. It is recommended to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Are trial sizes of this kit available?
Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted.
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Can individual components of this kit be purchased separately?
Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.