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Inosine Fluorometric Assay Kit

Catalog #: K712
$445.00

Product Details

Cat # +Size K712-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 535/587 nm)
Species Reactivity Mammalian
Applications This assay has a detection limit of of approximately 100 pmol inosine/well.
Features & Benefits • Simple procedure; takes ~ less than 40 minutes
• Fast and convenient
Kit Components • Inosine Assay Buffer
• PicoProbe™
• Converter Enzyme
• Developer Enzyme
• Inosine Substrate Mix
• Inosine Standard (10 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Inosine is commonly found in tRNAs and is essential for proper translation of the genetic code in wobble base pairs. Knowledge of inosine metabolism has led to advances in immunotherapy in recent decades. Inosine and hypoxanthine are also potential markers of ATP catabolic-by-products from oxidative stress which follows acute cardiac ischemia. In BioVision’s Inosine Assay Kit, inosine is converted to hypoxanthine, which will react with the substrate mix and PicoProbe™ to generate fluorescence (Ex/Em = 535/587 nm) in presence of converter and developer enzymes. This assay has a detection limit of of approximately 100 pmol inosine/well.


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Can you detect inositol with this kit?
No, this kit can only detect Inosine.
Can the kit detect inosine bound to DNA structures?
Our kit can only assays for free Inosine and not inosine in a DNA structure.
Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.