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Inorganic Polyphosphate Assay Kit (Fluorometric)

To quantify the amount of Inorganic Polyphosphate in samples
Catalog #: K2025
$515.00

Product Details

Size 100 assay
Detection Method Fluorescence (Ex/Em = 415/550 nm)
Species Reactivity Mammalian
Applications Determination of Poly P in different biological samples
Features & Benefits • A convenient tool for detecting Inorganic polyphosphate in various biological samples
• Sensitive
• Rapid
Kit Components • Poly P Assay Buffer
• Poly P Extraction Buffer
• Poly P Standard (45 mer)
• Poly P Dye
• RNase
• DNase
• Proteinase K
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Inorganic polyphosphate (Poly P) is a linear polymer of hundreds of orthophosphate (Pi) residues linked by high-energy, phosphoanhydride bonds. Poly P is ubiquitous and can be found in all living organisms from bacteria to mammals. Poly P has many important cellular functions. In microorganisms, Poly P plays an important role in virulence, biofilm formation, motility, quorum sensing, stress response and survival during nutrient deficiency. In mammals, it is important for blood coagulation, calcium precipitation, immune response, apoptosis, signal transduction and mitochondrial metabolism. Poly P also plays an important role in cancer cell proliferation. The traditional assay for Poly P quantification uses radioisotope methods, which are not convenient to perform. BioVision’s Inorganic Polyphosphate Assay Kit is a simple assay to quantify the amount of Poly P in various biological samples. The assay uses a fluorescent dye that forms a complex with Poly P present in the samples and the fluorsecent complex is measured at Ex/Em = 415/550 nm. The fluorescent signal is proportional to the Poly P concentration in the samples. BioVision’s Inorganic Polyphosphate Assay Kit is rapid, sensitive and a convenient tool for detecting Poly P. It can detect as low as 50 pmole under the assay conditions.


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Is there any limit on the length of the polyphosphate the kit can detect?
There is no maximum limit on the length for the detection. The longer the length, the better the detection as more fluorescent dyes are bound to the sample.