Product Details
Highlights | Applications: • The immobilized catalase beads can be used to eliminate or reduce sample background generated from hydrogen peroxide in assays that also use hydrogen peroxide for signal detection. Centrifugation to remove the immobilized catalase beads will prevent interference of catalase in assays that use hydrogen peroxide/HRP based mechanisms for detection. • If unwanted interfering substances are removed during sample prep by oxidation, the reaction can be carried out to completion with simultaneous removal of hydrogen peroxide User Supplied Reagents and Equipments: • 96-well polypropylene plate • Centrifuge for 96-well plates Protocol 1. Mix beads to generate homogeneous 50% suspension. 2. Add 10 µl of the 50 % slurry of the immobilized catalase beads to 50 µl sample of interest. 3. Incubate the sample at room temperature for fifteen minutes. 4. Spin down the sample at 1000 x g for a minute at RT to remove the beads. 5. Collect the supernatant and use for downstream analyses. |
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Storage Conditions | 4°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |
Details
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