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IL36B (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of IL36B

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5058
$725.00

Product Details

Cat # +Size E5058-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of IL36B in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Detection range: 31.25-2000 pg/ml
● Highly sensitive and specific
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Recovery Rate: 87-101% for serum, 86-96% for EDTA plasma and 86-100% for heparin plasma
● Sensitivity: 18.75 pg/ml
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (2000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Interleukin‑36 beta (IL36B) belongs to the IL‑1 family of proteins (1 ‑ 3, 6). IL36B binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Cells expressing IL-36 beta include resting and activated monocytes and B cells. The receptor for IL-36 beta is a blend of IL-1 Rrp2 and IL-1 RAcP. Recombinant IL-36 beta stimulates processes involving NF-kB and MAPK in an IL-1 Rrp2-dependent manner. In primary human joint cells (synovial fibroblasts and articular chondrocytes) IL-36β exerts proinflammatory effects, but serum levels of IL-36β do not correlate with joint inflammation. BioVision’s IL36B (Human) ELISA Kit is used for the quantitative detection of IL36B in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of IL36B in the sample.


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