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IL36A (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of IL36A

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5057
$725.00

Product Details

Cat # +Size E5057-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of IL36A in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Detection range: 0.156-10 ng/ml
● Sensitivity: 0.094 ng/ml
● Highly sensitive and specific
● Recovery Rate: 88-98% for serum, 85-103% for EDTA plasma and 85-104% for heparin plasma
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (10 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Interleukin-36 alpha (IL36A) is a novel cytokine which belongs to the IL-1 family that is involved in the development of inflammatory diseases. Cells reported to express IL36A includes monocytes, B cells and T cells (1, 4). IL36A binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. IL36A levels are elevated in the serum and cerebrospinal fluid of patients with neuromyelitis optica spectrum disorder and correlate with disease activity. Thus, IL-36A act as a novel biomarker for monitoring disease severity in Neuromyelitis optica spectrum disorder. BioVision’s IL36A (Human) ELISA Kit is used for the quantitative detection of IL36A in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of IL36A in the sample.


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