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IL17B (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of IL17B

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5060
$725.00

Product Details

Cat # +Size E5060-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of IL17B in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Highly sensitive and specific
● Recovery Rate: 87-105% for serum, 85-105% for EDTA plasma and 92-102% for heparin plasma
● Detection range: 15.625-1000 pg/ml
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Sensitivity: 9.375 pg/ml
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (1000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Interleukin-17B (IL17B) is a disulfide-linked homodimer of two 161 amino acid polypeptide chains. It belongs to the IL-17 family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. IL-17B involves in several physiological and pathological processes including embryonic development, tissue regeneration, inflammation and autoimmune diseases and tumors. IL-17B plays crucial roles in the pathogenesis of inflammatory arthritis, community-acquired pneumonia, systemic lupus erythematosus and rheumatoid arthritis. Among inflammatory mediators, a growing body of evidence emphasizes the contribution of the interleukin 17 cytokine family in malignant diseases. BioVision’s IL17B (Human) ELISA Kit is used for the quantitative detection of IL17B in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of IL17B in the sample.


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