Product Details
| Alternate Name | IL-7, Interleukin-7 |
|---|---|
| Gene Symbol | IL7 |
| Gene ID | 3574 |
| Accession # | P13232 |
| Source | E. coli |
| Appearance | Lyophilized protein |
| Physical Form Description | Lyophilized from a concentrated solution in water containing 1X PBS pH 7.4 |
| Molecular Weight | 17.4 kDa |
| Purity by SDS-PAGE | ≥98% |
| Purity by | ≥98% |
| Endotoxin Level | <0.1 ng/μg |
| Biological Activity | The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is <0.5 ng/ml, corresponding to a specific activity of >2 x 10⁶ units/mg. |
| Reconstitution Instructions | Reconstitute in H₂O to a concentration of 0.1 mg/ml, pipet up-and-down to dissolve. The solution can be diluted into other buffered solutions or stored at –20°C for future use. |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not to be used in humans |
Details
IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive. Recombinant human IL-7 is a 17.4 kDa protein containing 153 amino acid residues.
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Staron, M. et al. gp96, an Endoplasmic Reticulum Master Chaperone for Integrins and Toll-Like Receptors, Selectively Regulates Early T and B Lymphopoiesis. Blood 2010 115: 2380-2390.
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