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IL-34 (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of IL-34

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5056
$725.00

Product Details

Cat # +Size E5056-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of IL-34 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Sensitivity: 9.375 pg/ml
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Highly sensitive and specific
● Recovery Rate: 86-97% for Serum, 85-102% for EDTA plasma and 85-99% for Heparin plasma
● Detection range: 15.625-1000 pg/ml
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (1000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Interleukin-34 (IL-34) belongs to the short-chain helical hematopoietic cytokine family that promotes the proliferation, survival and differentiation of monocytes and macrophages. It specifically binds CD14+ monocytes, promotes survival and proliferation of human peripheral blood monocytes, and stimulates macrophage colony formation by human bone marrow cells. Clinical studies demonstrated that IL-34 levels significantly increase in the circulation and synovial fluid of patients with rheumatoid arthritis and knee osteoarthritis. Hence it is used as a promising clinical biomarker and therapeutic target for inflammatory arthritis. BioVision’s IL-34 (Human) ELISA Kit is used for the quantitative detection of IL-34 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of IL-34 in the sample.


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