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IL-31 (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of IL-31

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5066
$725.00

Product Details

Cat # +Size E5066-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of IL-31 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Highly sensitive and specific
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Sensitivity: 4.688 pg/ml
● Recovery Rate: 89-104% for serum, 86-98% for EDTA plasma and 85-105% for heparin plasma
● Detection range: 7.813-500 pg/ml
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (500 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Interleukin-31 (IL-31) is a cytokine belongs to the gp130/IL-6 cytokine family with an anti-parallel four-helix bundle structure. It is an inflammatory cytokine produced by activated CD4+ T lymphocytes, in particular activated TH2 helper cells, mast cells, macrophages, and dendritic cells. It’s major sites of action are the skin, lung, intestine and the nervous system. Hence its main role is to facilitate cell-mediated immunity against pathogens. IL-31 plays a role in the promotion of allergic skin disorders such as atopic dermatitis, or eczema and in regulating other allergic diseases, such as asthma. IL-31 acts as a novel diagnostic marker of allergic diseases. BioVision’s IL-31 (Human) ELISA Kit is used for the quantitative detection of IL-31 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of IL-31 in the sample.


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