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IL-22 (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for quantitative measurement of IL-22

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5063
$725.00

Product Details

Cat # +Size E5063-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of IL-22 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Highly sensitive and specific
● Detection range: 15.625-1000 pg/ml
● Sensitivity: 9.375 pg/ml
● Recovery Rate: 85-99% for serum, 85-102% for EDTA plasma and 86-102% for heparin plasma
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (1000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Interleukin-22 (IL-22) belongs to the IL-10 family cytokine that is produced by T helper 17 cells, γδ T cells, NKT cells, and innate lymphoid cells (ILCs). IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP. It is involved in the development of lymphocytes and serves as a rapid and early source of the effector cytokines that are released in response to pathogen-induced changes in the microenvironment. IL-22 has been increasingly recognized as a promising therapeutic option for various types of diseases. BioVision’s IL-22 (Human) ELISA Kit is used for the quantitative detection of IL-22 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of IL-22 in the sample.


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