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IL-21 (Human) ELISA Kit

A highly sensitive ELISA kit for the quantitative measurement of IL-21

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5052
$725.00

Product Details

Cat # +Size E5052-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of IL-21 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Recovery Rate: 88-98% for Serum, 88-104% for EDTA plasma and 89-96% for Heparin plasma
● Highly sensitive and specific
● Detection range: 62.5-4000 pg/ml
● Sensitivity: 37.5 pg/ml
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (4000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Interleukin-21 (IL-21) is a cytokine with broad pleiotropic actions that affect the differentiation and function of lymphoid and myeloid cells. IL-21 regulates both innate and adaptive immune responses and also exerts major effects on inflammatory responses that promote the development of autoimmune diseases and inflammatory disorders. IL-21 is expressed in activated human CD4+ T cells but not in most other tissues. In addition, IL-21 expression is up-regulated in Th2 and Th17 subsets of T helper cells, as well as T follicular cells. Regulation of the expression levels of IL-21 and IL-21R and blocking of their signal transduction pathways with blockers may be new treatment options for autoimmune diseases. BioVision’s IL-21 (Human) ELISA Kit is used for the quantitative detection of IL-21 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of IL-21 in the sample.


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