Product Details
| Cat # +Size | E4831-100 |
|---|---|
| Size | 96 assays |
| Detection Method | Sandwich ELISA, Absorbance (450 nm) |
| Species Reactivity | Rat |
| Applications | A Sandwich ELISA kit for the quantitative measurement of rat IL-2 in serum, plasma and other biological fluids. |
| Features & Benefits | • Detection Range: 31.25-2000 pg/mL
• Precision: Coefficient of variation is < 10% • Specificity: This kit recognizes Rat IL-2 in samples. No Significant cross-reactivity or interference between Rat IL-2 and analogues was observed. • Sensitivity: 18.75 pg/mL |
| Kit Components | • Micro ELISA Plate
• Reference Standard • Biotinylated Detection Ab (100x) • HRP Conjugate (100x) • Reference Standard & Sample Diluent • Biotinylated Detection Antibody Diluent • HRP Conjugate Diluent • Wash Buffer (25X) • Substrate Reagent • Stop Solution • Plate Sealer |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Interleukin-2 (Il-2) is produced by T-cells in response to antigenic or mitogenic stimulation. It is is required for T-cell proliferation and other activities crucial to regulation of the immune response. IL-2 can also stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. BioVision’s IL-2 (Rat) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Rat IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-2 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat IL-2. The concentration of Rat IL-2 in the samples can be calculated by comparing the OD of the samples to the standard curve.
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