IL-2 (Rat) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of rat IL-2 in serum, plasma and other biological fluids.
Catalog #: E4831 | abID:

Product Details

Cat # +Size E4831-100
Size 96 assays
Detection Method Sandwich ELISA, Absorbance (450 nm)
Species Reactivity Rat
Applications A Sandwich ELISA kit for the quantitative measurement of rat IL-2 in serum, plasma and other biological fluids.
Features & Benefits • Detection Range: 31.25-2000 pg/mL
• Precision: Coefficient of variation is < 10%
• Specificity: This kit recognizes Rat IL-2 in samples. No Significant cross-reactivity or interference between Rat IL-2 and analogues was observed.
• Sensitivity: 18.75 pg/mL
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Interleukin-2 (Il-2) is produced by T-cells in response to antigenic or mitogenic stimulation. It is is required for T-cell proliferation and other activities crucial to regulation of the immune response. IL-2 can also stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. BioVision’s IL-2 (Rat) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Rat IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-2 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat IL-2. The concentration of Rat IL-2 in the samples can be calculated by comparing the OD of the samples to the standard curve.

Why buy BioVision Products?
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.