IL-2 (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of human IL-2 in serum, plasma and other biological fluids.
Catalog #: E4829 | abID:

Product Details

Cat # +Size E4829-100
Size 96 assays
Detection Method Sandwich ELISA, Absorbance (450 nm)
Species Reactivity Human
Applications • A Sandwich ELISA kit for the quantitative measurement of human IL-2 in serum, plasma and other biological fluids.
Features & Benefits • Precision: Coefficient of variation is < 10%.
• Sensitivity: 4.69 pg/mL
• Detection Range: 7.81-500 pg/mL
• Specificity: This kit recognizes Human IL-2 in samples. No Significant cross-reactivity or interference between Human IL-2 and analogues was observed.
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Interleukin-2 (Il-2) is produced by T-cells in response to antigenic or mitogenic stimulation. It is is required for T-cell proliferation and other activities crucial to regulation of the immune response. IL-2 can also stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. BioVision’s IL-2 (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-2. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-2 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IL-2. The concentration of Human IL-2 in the samples can be calculated by comparing the OD of the samples to the standard curve.


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