Product Details
| Cat # +Size | E4832-100 |
|---|---|
| Size | 96 assays |
| Detection Method | Sandwich ELISA, Absorbance (450 nm) |
| Species Reactivity | Human |
| Applications | A Sandwich ELISA kit for the quantitative measurement of human IL-15 in Serum, plasma and other biological fluids. |
| Features & Benefits | • Specificity: This kit recognizes Human IL-15 in samples. No Significant cross-reactivity or interference between Human IL-15 and analogues was observed.
• Precision: Coefficient of variation is < 10%. • Sensitivity: 9.38 pg/mL • Detection Range: 15.63-1000 pg/mL |
| Kit Components | • Micro ELISA Plate
• Reference Standard • Biotinylated Detection Ab (100x) • HRP Conjugate (100x) • Reference Standard & Sample Diluent • Biotinylated Detection Antibody Diluent • HRP Conjugate Diluent • Wash Buffer (25X) • Substrate Reagent • Stop Solution • Plate Sealer |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of the IL2 receptor, including IL-2RB and probably IL-2RG but not IL-2RA. IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection. BioVision’s IL-15 (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-15. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human IL-15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-15 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IL-15. The concentration of Human IL-15 in the samples can be calculated by comparing the OD of the samples to the standard curve.
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