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IL-1α (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of IL-1α in human serum, plasma and other biological fluids.
Catalog #: E4802
$525.00

Product Details

Size 96 assay
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications in vitro quantitative measurement of IL-1α in human serum, plasma and other biological fluids.
Features & Benefits • Detection Range: 3.91-250 pg/mL
• Precision: Coefficient of variation is < 10%
• Specificity: No Significant cross-reactivity or interference between Human IL-1α and analogues was observed.
• Sensitivity: 2.35 pg/mL
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

IL-1α is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. BioVision’s IL-1α (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1α. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-1α bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IL-1 α. The concentration of Human IL-1α in the samples can be calculated by comparing the OD of the samples to the standard curve.


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