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IGFBP-3 (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of IGFBP-3 in human serum, plasma, tissue lysates, and other biological fluids
Catalog #: E4884
$695.00

Product Details

Cat # +Size E4884-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is designed to quantitatively measure amount of IGFBP-3 in human serum, plasma, tissue lysates and other biological fluids
Features & Benefits • Recovery range: between 85 - 105% for normal human serum and plasma samples
• This Sandwich ELISA is highly sensitive and highly specific for the detection of IGFBP-3 in human samples. There is no significant cross-reactivity or interference between IGFBP-3 and analogues
• Detection range: 0.391 - 25 ng/ml
• Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• Sensitivity: 0.234 ng/ml
Kit Components • Micro ELISA plate
• Standard (Lyophilized) (25 ng)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (25X)
• Plate Sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Insulin-like growth factor binding protein-3 (IGFBP-3) is a 264 amino acid peptide that belongs to the family of IGF-binding proteins. It is expressed in multiple tissues. One of the main functions of IGFBP-3 is that it regulates the transport, bioavailability, and half-life of insulin-like growth factors, especially IGF-1. IGFBP-3 is inversely correlated with cancer. Increased levels of IGFBP-3 in the serum inhibit proliferation and promote apoptosis of cancer cells, thus serving as a potential target for cancer research. BioVision’s IGFBP-3 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of IGFBP-3 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off with Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of IGFBP-3 in the sample or standard.


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