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IFNE (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of IFNE

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5061
$750.00

Product Details

Cat # +Size E5061-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of IFNE in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Sensitivity: 9.375 pg/ml
● Detection range: 15.625-1000 pg/ml
● Recovery Rate: 96-102% for serum, 92-97% for EDTA plasma and 86-100% for heparin plasma
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Highly sensitive and specific
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (1000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Interferon epsilon (IFNE) is type I interferon which stands out through its unusual expression profile and differing regulation compared to classic type I interferons such as interferon alpha and interferon beta. Type I interferon is required for maintaining basal levels of IFN-regulated genes, including 2'-5'-oligoadenylate synthetase, IRF7 and ISG15, in the female reproductive tract. It directly mediates protection against viral and bacterial genital infections. It is expressed constitutively in the female reproductive tract, and contributes to protection in models of sexually transmitted infections. BioVision’s IFNE (Human) ELISA Kit is used for the quantitative detection of IFNE in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of IFNE in the sample.


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