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Hygromycin B

An antibiotic and a selection agent

WARNING: This product can expose you to chemicals including Hygromycin B, which is [are] known to the State of California to cause birth defects or other reproductive harm.  For more information go to
Catalog #: 2589
SKU-Size Size Price Qty
2589-500 500 mg
2589-1000 1 g
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Product Details

Alternate Name (3'R,3aS,4S,4'R,5'R,6R,6'R,7S,7aS)-4-[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
Appearance Off-white to light tan powder
CAS # 31282-04-9
Molecular Formula C₂₀H₃₇N₃O₃
Molecular Weight 527.52
Purity ≥90% by HPLC
Solubility H₂O (~ 50 mg/ml)
InChi InChI=1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1
PubChem CID 35766
MDL Number MFCD06795479
Handling Protect from light and moisture
Storage Conditions 2-8ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus that is routinely used as a selection agent for cells that contain hph or hyg Hygromycin resistant gene. Hygromycin B is thought to inhibit protein synthesis by strengthening the interaction of peptidyl-tRNA in the ribosomal acceptor (“A”) site thereby preventing translocation of by EF-2 (elongation Factor-2). Hygromycin B is effective on most bacteria, fungi and higher eukaryotes.

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