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Human Whole Blood Monocyte Isolation Kit

Simple, one step assay to isolate monocytes

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Catalog #: K933

Product Details

Cat # +Size K933-30
Size 30 ml isolation
Kit Summary • Isolation of Monocytes from human whole blood.
• Determination of cell viability and also contamination from RBCs in isolated cell fraction.
Species Reactivity Human
Applications • Isolation of Monocytess from human whole blood.
• Determination of cell viability and also contamination from RBCs in isolated cell fraction.
Features & Benefits • PBMC high yields (≥5 x106 cells/ml)
• By using the Viability Stain, it was proven that 99% of the cells are viable and the isolated fraction contains low red blood cell counts (≤ 3%)
Kit Components • Monocyte Isolation Buffer (MIB)
• Monocyte Density Gradient Media (MDGM)
• 54% Density media
• Viability Stain
• Blunt-end needle 18 G; 1.5 in. (Sterile)
• Giemsa Stain
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Monocytes are a type of white blood cell (leukocyte) and the largest of the immune cells. They are produced in the bone marrow and then migrate to the blood stream where they comprise 3-8% of all leukocytes. Depending on the clincial presentation of disease, monocytes will migrate to a specific tissue and then differentiate into a tissue-specific dendritic cell or a macrophage. Monocytes are easily identified by the large amoeboid shape, unilobar nucleus and vacuolated cytoplasm. They serve three important roles: phagocytosis, presentation of antigen to T cells which subsequently results in T cell clonal expansion, and the production of cytokines. BioVision’s human Whole Blood Monocyte Isolation Kit allows for in vitro isolation of intact, viable monocytes. The kit enables the isolation of ≥ 5 x106 monocytes/ml yielding approximately 80% of the total monocytes present in 1 ml of whole blood. The viability stain (included) used to identify living monocytes indicates that more than 99% of the cells in the isolated fraction are viable. The isolated fraction is pure - it contains more than 95% monocytes with less than 3% red blood cell counts.

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How are monocytes separated from the lymphocytes using Human Whole Blood Monocyte Isolation Kit as both of these cell types have similar densities?
Although both monocytes and lymphocytes have similar densities, separation occurs because these cells migrate at differing rates through the gradient to reach their equilibration point. The contamination from lymphocytes using the density gradient method is least expected.