Human recombinant Ubiquitin Activating Enzyme E1

First enzyme involved in ubiquitin conjugation pathway, conjugating ubiquitin to the substrate.
Catalog #: 6429 | abID: ab285724

Product Details

abID ab285724
Cat # +Size 6429-50
Size 50 μg
Alternate Name UBE1, UBA1, POC20, A1S9T, A1ST, AMCX1, GXP1, SMAX2
Gene Symbol UBA1
Gene ID 7317
Accession # P22314
Source E.coli
Appearance Liquid
Physical Form Description In 50 mM Tris (pH 8.0), 100 mM NaCl and 10% glycerol.
Molecular Weight 118 kDa
Purity by SDS-PAGE ≥95%
Biological Activity Typical enzyme concentration of 5 nM – 100 nM is used for in vitro conjugation, depending on conditions.
Handling Centrifuge the vial prior to opening.
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not to be used in humans


UBE1, the ubiquitin activating enzyme, is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate.

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