Product Details
| abID | ab51289 |
|---|---|
| Cat # +Size | 6302-100 |
| Size | 100 μg |
| Alternate Name | Protein tyrosine phosphatase, non-receptor type 6 isoform 1, PTPN6, HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1L, SHP1. |
| Gene Symbol | PTPN6 |
| Gene ID | 5777 |
| Accession # | P29350 |
| Source | E. coli |
| Appearance | Liquid |
| Physical Form Description | 1 mg/ml solution in 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 2 mM β-mercaptoethanol, 1 mM EDTA, 20% glycerol. |
| Molecular Weight | 34.3 kDa (300 aa) |
| Purity by SDS-PAGE | ≥95% |
| Endotoxin Level | < 1.0 EU per 1 μg of protein (determined by LAL method) |
| Biological Activity | >5,000 units/mg of SHP-1. |
| Amino Acid Sequence | MGFWEEFESL QKQEVKNLHQ RLEGQRPENK GKNRYKNILP FDHSRVILQG RDSNIPGSDY INANYIKNQL LGPDENAKTY IASQGCLEAT VNDFWQMAWQ ENSRVIVMTT REVEKGRNKC VPYWPEVGMQ RAYGPYSVTN CGEHDTTEYK LRTLQVSPLD NGDLIREIWH YQYLSWPDHG VPSEPGGVLS FLDQINQRQE SLPHAGPIIV HCSAGIGRTG TIIVIDMLME N |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not to be used in humans |
Details
The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). The recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid (Met) is attached at N-terminus.
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