Human Recombinant SHP-1

A signaling molecule that regulates a variety of cellular processes
Catalog #: 6302 | abID: ab51289

Product Details

abID ab51289
Cat # +Size 6302-100
Size 100 μg
Alternate Name Protein tyrosine phosphatase, non-receptor type 6 isoform 1, PTPN6, HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1L, SHP1.
Gene Symbol PTPN6
Gene ID 5777
Accession # P29350
Source E. coli
Appearance Liquid
Physical Form Description 1 mg/ml solution in 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 2 mM β-mercaptoethanol, 1 mM EDTA, 20% glycerol.
Molecular Weight 34.3 kDa (300 aa)
Purity by SDS-PAGE ≥95%
Endotoxin Level < 1.0 EU per 1 μg of protein (determined by LAL method)
Biological Activity >5,000 units/mg of SHP-1.
Amino Acid Sequence MGFWEEFESL QKQEVKNLHQ RLEGQRPENK GKNRYKNILP FDHSRVILQG RDSNIPGSDY INANYIKNQL LGPDENAKTY IASQGCLEAT VNDFWQMAWQ ENSRVIVMTT REVEKGRNKC VPYWPEVGMQ RAYGPYSVTN CGEHDTTEYK LRTLQVSPLD NGDLIREIWH YQYLSWPDHG VPSEPGGVLS FLDQINQRQE SLPHAGPIIV HCSAGIGRTG TIIVIDMLME N
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). The recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid (Met) is attached at N-terminus.


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