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Product Details
| Alternate Name | M-CK, Creatine kinase M chain, CKM; Creatine phosphokinase M-type; CPK-M |
|---|---|
| Accession # | AAH07462.1 |
| Source | E. coli |
| Appearance | Liquid |
| Physical Form Description | Liquid |
| Molecular Weight | The recombinant human CKMM consists of 396 amino acids and has a molecular mass of 44.9 KDa. It migrates as a ~45 KDa band in SDS-PAGE under reducing conditions. |
| Purity by SDS-PAGE | ≥ 90% as determined |
| Amino Acid Sequence | A DNA sequence encoding the human CKM (AAH07462.1) (Met1-Lys381) was expressed with a polyhistidine tag at the N-terminus. |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20ºC |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
CK-MM is an isozyme of creatine kinase (CK). Cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). CK-MM is mainly expressed in skeletal muscle and can also be detected in heart muscle. It catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). Creatine kinase is measured as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, etc.
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