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Human Prothrombin ELISA Kit

For the quantitative measurement of prothrombin in human plasma and serum samples
Catalog #: E4913
$695.00

Product Details

Cat # +Size E4913-100
Size 96 assays
Kit Summary BioVision’s Prothrombin ELISA Kit is a sandwich ELISA for the quantitative measurement of prothrombin in human plasma and serum samples.
Detection Method Absorbance at 450 nm
Species Reactivity Human
Applications Quantitative determination of prothrombin in plasma or serum samples
Features & Benefits The kit offers an easy, reliable method to quantitate prothrombin in human biological samples.
Kit Components ● Pre-coated 96 well Strip Plate
● Prothrombin Standard
● HRP-Conjugate
● Prothrombin Antibody
● TMB Substrate
● Stop Solution
● Prothrombin Assay Buffer
● Wash Buffer (10X)
● Plate Sealers
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Prothrombin, also known as coagulation factor II, is a vitamin K-dependent plasma protein, which is synthesized in the liver. It is the precursor of thrombin and is proteolytically cleaved by the prothrombinase complex to form active thrombin in the blood coagulation pathway. Thrombin catalyzes many coagulation-related reactions including conversion of fibrinogen to fibrin, platelet activation and aggregation etc. Activation of prothrombin to active thrombin is a crucial step in physiological and pathological coagulation. Various rare diseases involving prothrombin includes dysprothrombinemia, hypoprothrombinemia etc. Additionally, prothrombin deficiency, also known as Factor II deficiency, is an inherited blood disorder that affects the ability of blood to clot properly thereby resulting in prolonged bleeding. A prothrombin time (PT) test is used clinically to measure the time it takes blood to clot. BioVision’s Prothrombin ELISA Kit is a sandwich ELISA for the quantitative measurement of prothrombin in human plasma and serum samples. In this assay, standards or samples (plasma or serum) are incubated in the ELISA plate coated with anti-prothrombin antibody. After incubation, the wells are washed and another prothrombin antibody & HRP conjugated antibody is added, which binds to the prothrombin captured in the wells. Following incubation, the wells are washed and the bound prothrombin is detected by the addition of TMB substrate, which generates a blue-colored product. Finally, the reaction is terminated using a stop solution thereby generating a yellow colored product. The intensity of the yellow color is proportional to the amount of prothrombin in samples. The kit offers an easy, reliable method to quantitate prothrombin in human biological samples.


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