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Product Details
| Cat # +Size | K926-100 |
|---|---|
| Size | 100 assays |
| Detection Method | Absorbance (550 nm) |
| Species Reactivity | Human |
| Applications | ELISA |
| Features & Benefits | • Easy, convenient and time-saving method to assay for human ?IG-H3 • The minimum detectable dose of ?IG-H3 is typically less than 25 pg/ml. • This ELISA kit shows no cross-reactivity with the rm?IGH3. The inter assay reproducibility CV is <12% and the intra assay CV is <10%. • Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed. |
| Kit Components | • βIG-H3 Microplate: 96 wells (12 strips x 8 wells) coated with anti-human βIG-H3. • Wash Buffer Concentrate (20x): 25 ml of 20x concentrated solution. • Standards: 2 vials of recombinant human βIG-H3. • Assay Diluent A: 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. • Assay Diluent B: 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. • Detection Antibody βIG-H3: 2 vial of biotinylated anti-human βIG-H3 (each vial is enough to assay half microplate). • HRP-Streptavidin Concentrate: 200 µl 300x concentrated HRP-conjugated streptavidin. • TMB One-Step Substrate Reagent: 12 ml of 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffer solution. • Stop Solution: 8 ml of 0.2 M sulfuric acid. |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
BioVision’s Human βIG-H3 (also known as Kerato-epithelin, RGDCAP) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human βIG-H3 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human βIG-H3 coated on a 96-well plate. Standards and samples are pipetted into the wells and βIG-H3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human βIG-H3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of βIG-H3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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