Product Details
Cat # +Size | K926-100 |
---|---|
Size | 100 assays |
Detection Method | Absorbance (550 nm) |
Species Reactivity | Human |
Applications | ELISA |
Features & Benefits | • Easy, convenient and time-saving method to assay for human ?IG-H3 • The minimum detectable dose of ?IG-H3 is typically less than 25 pg/ml. • This ELISA kit shows no cross-reactivity with the rm?IGH3. The inter assay reproducibility CV is <12% and the intra assay CV is <10%. • Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed. |
Kit Components | • βIG-H3 Microplate: 96 wells (12 strips x 8 wells) coated with anti-human βIG-H3. • Wash Buffer Concentrate (20x): 25 ml of 20x concentrated solution. • Standards: 2 vials of recombinant human βIG-H3. • Assay Diluent A: 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. • Assay Diluent B: 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. • Detection Antibody βIG-H3: 2 vial of biotinylated anti-human βIG-H3 (each vial is enough to assay half microplate). • HRP-Streptavidin Concentrate: 200 µl 300x concentrated HRP-conjugated streptavidin. • TMB One-Step Substrate Reagent: 12 ml of 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffer solution. • Stop Solution: 8 ml of 0.2 M sulfuric acid. |
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |
Details
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