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Human βIG-H3 ELISA Kit

Catalog #: K926
$645.00

Product Details

Cat # +Size K926-100
Size 100 assays
Detection Method Absorbance (550 nm)
Species Reactivity Human
Applications ELISA
Features & Benefits • Easy, convenient and time-saving method to assay for human ?IG-H3
• The minimum detectable dose of ?IG-H3 is typically less than 25 pg/ml.
• This ELISA kit shows no cross-reactivity with the rm?IGH3. The inter assay reproducibility CV is <12% and the intra assay CV is <10%.
• Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.
Kit Components • βIG-H3 Microplate: 96 wells (12 strips x 8 wells) coated with anti-human βIG-H3.
• Wash Buffer Concentrate (20x): 25 ml of 20x concentrated solution.
• Standards: 2 vials of recombinant human βIG-H3.
• Assay Diluent A: 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent.
• Assay Diluent B: 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent.
• Detection Antibody βIG-H3: 2 vial of biotinylated anti-human βIG-H3 (each vial is enough to assay half microplate).
• HRP-Streptavidin Concentrate: 200 µl 300x concentrated HRP-conjugated streptavidin.
• TMB One-Step Substrate Reagent: 12 ml of 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffer solution.
• Stop Solution: 8 ml of 0.2 M sulfuric acid.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

BioVision’s Human βIG-H3 (also known as Kerato-epithelin, RGDCAP) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human βIG-H3 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human βIG-H3 coated on a 96-well plate. Standards and samples are pipetted into the wells and βIG-H3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human βIG-H3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of βIG-H3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


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