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Human Hexokinase (HK) Inhibitor Screening Kit (Colorimetric)

Market's first and only kit for screening Human Hexokinase Inhibitors. Simple and HTP compatible. Screen within 30 min.
Catalog #: K713

In stock

$555.00

Product Details

Cat # +Size K713-100
Size 100 assays
Detection Method Absorbance at 450 nm
Species Reactivity Human
Applications Screening/characterizing/studying potential inhibitors of human Hexokinase
Features & Benefits Simple and High throughput adaptable
Colorimetric Detection
Screen within 30 min
Kit Components • HK Assay Buffer
• HK Substrate
• HK Coenzyme
• HK Converter
• HK Developer
• Hexokinase II (human)
• HK Inhibitor Control (Bromopyruvic Acid)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Hexokinases (HK, EC: 2.7.1.1) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. The Hexokinase family phosphorylates glucose and generates glucose-6-phosphate for glycolysis. Four Hexokinase isoforms (HK-I, II, III and IV) are found in numerous species. HK-I, HK-II and HK-III have low affinity for glucose, one of their natural substrates, while HK-IV show high affinity for this monosaccharide. Recent studies have found increased hexokinase activity in various human metastatic tumors. Moreover, Hexokinase II (HKII) is the main isoform of the Hexokinases and is responsible for malignant phenotypes. HKII binds to the outer mitochondrial membrane via the Voltage-Dependent Anion Channel (VDAC), a Porin-like protein. HKII has become an attractive therapeutic target for its role in cancer metastasis. BioVision’s Human Hexokinase II (HKII) Inhibitor Screening kit uses the HKII’s ability to convert glucose into glucose-6-phosphate. Glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a probe that shows strong absorbance at 450 nm. In the presence of Bromopyruvic Acid, reactions are impeded, thus decreasing the rate and/or extent of generation of Hexokinase -dependent absorbance at OD 450 nm. Hexokinase Inhibitor Control is included to compare the efficacy of test inhibitors. This kit provides a sensitive, quick, and easy method for screening potential inhibitors of Hexokinase. The assay is high-throughput adaptable and can be performed in less than 30 min


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Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.