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Human GAPDH Inhibitor Screening Kit (Colorimetric)

To screen potential inhibitors of Human GAPDH
Catalog #: K2043
$545.00

Product Details

Cat # +Size K2043-100
Size 100 assays
Detection Method Absorbance 450 nm
Species Reactivity Human
Applications • Screening, characterizing & studying potential inhibitors of Human GAPDH
Features & Benefits • Simple, sensitive
• Quick and easy method for screening potential inhibitors of GAPDH
Kit Components • GAPDH Assay Buffer
• GAPDH Substrate
• GAPDH Developer
• Human GAPDH
• GAPDH Reconstitution Buffer
• GAPDH Inhibitor Control
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glucose 3-Phosphate Dehydrogenase (GAPDH; EC 1.2.1.12) is a cytosolic enzyme, which catalyzes the conversion of Glyceraldehyde 3-Phosphate to 1,3-Bisphosphoglycerate (BPG) with the reduction of NAD to NADH in the glycolytic pathway. GAPDH is a multifunctional protein involved in many intracellular processes such as apoptosis, membrane trafficking, ion metabolism, nuclear translocation etc. Although GAPDH is considered as housekeeping gene, increased GAPDH activity has been detected in various human cancers. Additionally, knockdown of GAPDH has been shown to decrease cell proliferation, cell migration and metastasis in in vitro experiments. Therefore, targeting this key enzyme is essential for developing novel therapeutics for treating cancer. In our GAPDH Inhibitor Screening Kit, Glyceraldehyde 3-Phosphate is oxidized by GAPDH to generate 1,3-Bisphosphoglycerate and NADH, which reduces the probe thereby generating a strong absorbance at 450 nm. In the presence of GAPDH inhibitors, the reactions are impeded, thus decreasing the rate and/or extent of generation of GAPDH-dependent absorbance at OD 450 nm. This kit provides a sensitive, quick, and easy method for screening potential inhibitors of GAPDH. GAPDH Inhibitor Control is included in the kit to compare the efficacy of test inhibitors. The assay is high-throughput adaptable and can be performed in less than 30 min.


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