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Human GAPDH Activity Assay Kit II

For monitoring GAPDH activity
Catalog #: K2047
$245.00

Product Details

Cat # +Size K2047-100
Size 100 assays
Kit Summary BioVision’s Human GAPDH Activity Assay Kit II provides a simple and sensitive method for monitoring GAPDH activity in various sample types. In this assay, GAPDH catalyzes GAP into BPG and an intermediate, which then reacts with the developer to form a colored product that is measured at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 10 mU/mL in a variety of samples.
Detection Method Absorbance (OD 450 nm)
Species Reactivity All
Applications • Analysis of glycolysis and pentose phosphate pathways
• Measurement of GAPDH activity in various tissues and cells
Features & Benefits • Simple, rapid & convenient
• Sensitive method for monitoring GAPDH activity in various sample types
Kit Components • GAPDH Assay Buffer
• GAPDH Substrate
• GAPDH Developer
• NADH Standard
• Human GAPDH Positive Control
• GAPDH Reconstitution Buffer
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH is a stable metabolic enzyme and is constitutively expressed. Therefore, it is considered as a housekeeping gene. Deregulation of GAPDH activity is associated with cell proliferation and carcinogenesis. Accurate detection of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology. BioVision’s Human GAPDH Activity Assay Kit II provides a simple and sensitive method for monitoring GAPDH activity in various sample types. In this assay, GAPDH catalyzes GAP into BPG and an intermediate, which then reacts with the developer to form a colored product that is measured at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 10 mU/mL in a variety of samples.


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