Product Details
abID | ab285844 |
---|---|
Alternate Name | KDR, CD309, FLK1, VEGFR, VEGFR2, kinase insert domain receptor |
Gene Symbol | VEGFR2 |
Gene ID | 3791 |
Accession # | P35968 |
Source | HEK293 cells |
Appearance | Lyophilized |
Physical Form Description | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
Molecular Weight | This protein with 6×his tag at C-terminus, and has a calculated MW of 84.1 kDa. DTT-reduced protein migrates as 100-110 kDa protein due to glycosylation. |
Purity by SDS-PAGE | ≥95% |
Endotoxin Level | <1 EU/μg by LAL method |
Biological Activity | Measured by its ability to inhibit the VEGF dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 9-20 ng/ml in the presence of 5 ng/ml rhVEGF165. |
Reconstitution Instructions | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
Handling | Centrifuge the vial prior to opening. |
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not to be used in humans |
Details
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