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Product Details
| Alternate Name | Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1. |
|---|---|
| Gene Symbol | TGFB1 |
| Gene ID | 7040 |
| Accession # | P01137 |
| Source | Human 293 cell expressed |
| Appearance | Lyophilized |
| Physical Form Description | Lyophilized from 50 mM sodium acetate, pH 4.5 and 350 mM NaCl. |
| Molecular Weight | 25 kDa, homodimer, nonglycosylated |
| Purity by SDS-PAGE | > 95% |
| Endotoxin Level | < 1.0 EU per 1 μg of protein |
| Biological Activity | ED₅₀ is typically 0.1 to 0.5 ng/mL. The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2). |
| Reconstitution Instructions | Reconstitute in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin. |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not to be used in humans |
Details
Transforming growth factor betas (TGFβ) mediate many cell-cell interactions that occur during embryonic development. Three TGFβs have been identified in mammals. TGF Beta 1, TGF Beta 2 and TGF Beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule. TGF-beta 1 is involved in hematopoiesis and endothelial differentiation.
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