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Human CellExp™ TFPI, human recombinant

A protein which reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa)
Catalog #: 7453
$220.00

Product Details

Cat # +Size 7453-10
Size 10 μg
Alternate Name TFPI, LACI, TFPI1, EPI, TFI
Gene Symbol TFPI
Gene ID 7035
Accession # P10646
Source HEK293 cells
Appearance Lyophilized
Physical Form Description Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Molecular Weight This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 30 kDa. The predicted N-terminus is Asp 29. DTT-reduced Protein migrates as 41-45 kDa due to glycosylation.
Purity by SDS-PAGE ≥95%
Endotoxin Level <1 EU/μg by LAL method
Biological Activity Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval – WRK (Dnp) - NH2. The IC50 value is < 0.35 nM, as measured in 100 μl reaction mixture containing 1.25 ng trypsin, 10 μM substrate, 50 mM Tris, 10
Reconstitution Instructions Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Tissue factor pathway inhibitor (TFPI) also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma protein inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N­terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C­terminal basic region. The first domain inhibits coagulation factor VIIa complexed to tissue factor (TF); The second domain inhibits factor Xa; The third K domain binds to heparin; The C­terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.


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