Product Details
abID | ab82279 |
---|---|
Alternate Name | soluble CD14, Cluster of differentiation 14, Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14 |
Gene Symbol | CD14 |
Gene ID | 929 |
Accession # | P08571 |
Source | HEK 293 cells |
Appearance | Lyophilized powder |
Physical Form Description | Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium phosphate, pH 7.5. |
Molecular Weight | ~35 kDa |
Purity by SDS-PAGE | ≥95% |
Endotoxin Level | < 0.1 ng/μg of protein (<1EU/μg). |
Biological Activity | Determined by the dose dependent activation of NF-kappaB in a RAW264 cell line based reporter system, using a sCD14 concentration range of 20 ng/ul to 200 ng/ul. The NF-kappaB activation is enhanced when the assay is done in the presence of 0.25 ng/ul to |
Reconstitution Instructions | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carri |
Amino Acid Sequence | TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QP |
Handling | Centrifuge the vial prior to opening. |
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not to be used in humans |
Details
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