Product Details
Alternate Name | MMP2, Matrix metalloproteinase-2, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, TBE-1, CLG4A, EC:3.4.24.24 |
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Gene Symbol | MMP-2 |
Gene ID | 4313 |
Accession # | P08253 |
Source | HEK 293 cells |
Appearance | Lyophilized powder |
Physical Form Description | Lyophilized |
Molecular Weight | This protein has 450 amino acids with a 6x His tag at C-terminus, and a calculated MW of 51 kDa. The N-terminal is Ala 20. The protein migrates to ~60 kDa under reducing conditions (SDS-PAGE) due to glycosylation. |
Purity by SDS-PAGE | ≥95% |
Biological Activity | Different amounts of activated MMP-2 were incubated with FRET-based substrate using BioVision’s MMP-9 Inhibitor Screening Kit (Fluorometric) (Cat. No. K844). The fluorescence (Ex/Em = 325/393 nm) was monitored over a 60 min time period. |
Reconstitution Instructions | Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex. |
Amino Acid Sequence | Ala 30 – Cys 660 |
Handling | Centrifuge the vial prior to opening. |
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not to be used in humans |
Details
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