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Human CellExp™ LIF, human recombinant

Affects cell growth and development
Catalog #: 7267
$310.00

Product Details

Cat # +Size 7267-10
Size 10 μg
Alternate Name LIF, CDF, DIA, HILDA, MLPLI
Gene Symbol LIF
Gene ID 3976
Accession # P15018
Source HEK 293 cells
Appearance Lyophilized powder
Physical Form Description Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular Weight Calculated MW of 19.9 kDa with Gly-Pro at the N- terminus. The predicted N-terminus is Ser 23. DTT-reduced Protein migrates as 35-45 kDa.
Purity by SDS-PAGE ≥95%
Endotoxin Level < 0.1 ng/μg of protein (<1EU/μg) by LAL method
Biological Activity Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 <0.1 ng/ml, corresponding to a specific activity of >1X10^7 units/mg.
Reconstitution Instructions Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.


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