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Human CellExp™ FOLR1, human recombinant

Mediates delivery of 5-methyltetrahydrofolate to the interior of cells
Catalog #: 7456
SKU-Size Size Price Qty
7456-10 10 μg
$180.00
7456-50 50 μg
$595.00
More Sizes Get Quote

Product Details

Alternate Name FOLR1, FBP, FOLR, FOLR-1, Folate-receptor-alpha
Gene Symbol FOLR1
Gene ID 2348
Accession # P15328
Source HEK293 cells
Appearance Lyophilized
Physical Form Description Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Molecular Weight This protein is fused with 6×his tag at the C-terminus and has a calculated MW of 25.4 kDa expressed. The predicted N-terminus is Arg 25. Protein migrates as 33-37 kDa in reduced SDS-PAGE resulting from glycosylation.
Purity by SDS-PAGE ≥95%
Endotoxin Level <1 EU/μg by LAL method
Biological Activity Measured by its binding ability in a functional ELISA. When Folic Acid Bovine Serum Albumin is coated at 5 μg/ml (100 μl/well), the concentration of rhFOLR1 that produces 50% of the optimal binding response is found to be approximately 0.35-1.8 nM.
Reconstitution Instructions Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Folate Receptor 1 (FOLR1) also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.


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