Human CellExp™ DKK3, human recombinant

Proposed as tumor suppressor gene and a marker for tumor blood vessels
Catalog #: 7243 | abID: ab155717

Product Details

abID ab155717
Cat # +Size 7243-50
Size 50 μg
Alternate Name DKK3, REIC, RIG
Gene Symbol DKK3
Gene ID 27122
Accession # Q9UBP4
Source HEK 293 cells
Appearance Lyophilized powder
Physical Form Description Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular Weight This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 37.6 kDa. The predicted N-terminus is Pro 23. DTT-reduced Protein migrates as 50-60 kDa due to glycosylation.
Purity by SDS-PAGE ≥95%
Endotoxin Level < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is typically 2.0-11.4 μg/ml.
Reconstitution Instructions Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Members of the Dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumor suppressor gene and a marker for tumor blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.


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