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Human CellExp™ CD33/SIGLEC-3

Human recombinant with Fc & His Tag
Catalog #: P1616
SKU-Size Size Price Qty
P1616-20 20 μg
$245.00
P1616-50 50 μg
$425.00
More Sizes Get Quote

Product Details

Alternate Name gp67; Siglec-3; CD33; Sialic acid binding Ig-like lectin 3; Myeloid cell surface antigen CD33
Gene ID 945
Accession # P20138; NP_001763.3
Source HEK293 cells
Appearance Lyophilized protein
Molecular Weight The protein migrates as a band at 70 kDa (Fc and 6×His tag at the C-terminus).
Purity by SDS-PAGE ≥ 97%
Endotoxin Level < 0.1 EU/μg of the protein as determined by LAL method.
Biological Activity The activity was measured by the binding ability in a functional ELISA. Immobilized Human CD33 at 2 μg/ml (100 μl/well) can bind Anti-Human CD33 Antibody in the linear range of 8-20 ng/ml.
Reconstitution Instructions Reconstitute to a concentration of 0.1-0.5 mg/ml in sterile distilled water.
Amino Acid Sequence aa Asp18-His259 of human protein. The target protein is expressed with the sequence (Asp18-His259) of human CD33/Siglec-3 fused with an Fc and 6×His tag at the C terminus.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

CD33 is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This single-pass type I membrane protein contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like Vtype (immunoglobulin-like) domain. It is considered myeloid-specific, but it can also be found on some lymphoid cells. It binds to α2-6- and α2-3-sialylated glycans and sialylated ligands. It acts as an inhibitory receptor since, when it is crosslinked, it becomes phosphorylated on its cytoplasmic ITIM sequence that recruits SHP-1 and SHP-2 phosphatases and negatively regulates cell activation in both myeloid cell lines and activated NK cells. It induces apoptosis in acute myeloid leukemia and is a target for the treatment of leukemia.


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