Human CellExp™ CD28, Human Recombinant

T-cell-specific surface glycoprotein CD28
Catalog #: 9237 | abID: ab286013

Product Details

abID ab286013
Alternate Name CD28, Tp44
Gene Symbol CD28
Gene ID 940
Accession # P10747
Source HEK 293 cells
Appearance Lyophilized
Physical Form Description Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
Molecular Weight 41 kDa (calculated), 55 kDa (observed)
Purity by SDS-PAGE > 95%
Formulation Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4) with 5% Trehalose
Reconstitution Instructions Reconstitute in ddH2O or sterile PBS, pH 7.4.
Specific Activity Human biotinylated CD80, Fc Tag (BioVision Cat. No. P1668) can bind to immobilized human CD28, Fc Tag at 1 µg/mL, 100 µL/well with a linear range of 0.03 – 0.81 µg/mL.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Human CD28 is composed of four exons encoding a protein of 220 amino acids that is expressed on the cell surface as a glycosylated, disulfide-linked homodimer of 44 kDa. Members of the CD28 family share a number of common features. These receptors consist of paired V-set immunoglobulin superfamily (IgSF) domains attached to single transmembrane domains and cytoplasmic domains that contain critical signaling motifs. The CD28 and CTLA4 ligands, CD80 and CD86, consist of single V-set and C1-set IgSF domains. The interaction of these costimulatory receptors with ligands is mediated through the MYPPPY motif within the receptor V-set domains. CD28 is expressed constitutively on almost all human CD4 T cells and approximately 50% of CD8 T cells. CD28 costimulation has diverse effects on T cell function, including biochemical events at the immunological synapse, downstream phosphorylation and other post-translational modifications, transcriptional changes, and cytoskeletal remodeling. At the most basic level, CD28 signals increase a cell’s glycolytic rate, allowing cells to generate the energy necessary for growth and proliferation.


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