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Product Details
| Cat # +Size | 7408-10 |
|---|---|
| Size | 10 μg |
| Alternate Name | CTSB, CPSB, APPS |
| Gene Symbol | CTSB |
| Gene ID | 1508 |
| Accession # | P07858 |
| Source | HEK293 cells |
| Appearance | Lyophilized |
| Physical Form Description | Lyophilized from 0.22 μm filtered solution in 50 mM Tris-HCl and 150 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
| Molecular Weight | This protein is fused with a 6×His tag at the C-terminus, has a calculated MW of 36.7 kDa (Pro) and 29 kDa (Mature). The predicted N-terminus is Arg18 (pro) or Phe74 (mature). DTT-reduced Protein migrates as 43 kDa and 34 kDa due to glycosylation. |
| Purity by SDS-PAGE | ≥95% |
| Endotoxin Level | <1 EU/μg by LAL method |
| Biological Activity | Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100 μl reaction mixture containing 25 mM MES, pH 5.0, 0.01 µg rhCathepsin B, and 10 µM reaction substrate. The specific activity is >2800 pmol/min/µg. |
| Reconstitution Instructions | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not to be used in humans |
Details
Cathepsin B (CTSB) also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L); In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.
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