Product Details
| Alternate Name | T-cell-specific surface glycoprotein CD28; CD antigen: TP44; Tp44; T-Cell-Specific Surface Glycoprotein; CD28 Molecule |
|---|---|
| Gene ID | 940 |
| Accession # | P10747 |
| Source | HEK 293 cells |
| Appearance | Lyophilized protein |
| Physical Form Description | Lyophilized powder |
| Molecular Weight | ~42 kDa (Human Fc Tag fused to C-terminus, Biotinylated) |
| Purity by SDS-PAGE | > 95% |
| Formulation | Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with 5% Trehalose |
| Amino Acid Sequence | Asn 19 - Pro 152 |
| Specific Activity | Biotinylated human CD28-Fc can bind to immobilized Human CD80, Fc Tag (Cat. No. P1668 coated at 1 µg/mL, 100 µL/well) with a linear range 0.01 – 0.27µg/ml. |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20 °C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Human CD28 is composed of four exons encoding a protein of 220 amino acids that is expressed on the cell surface as a glycosylated, disulfide-linked homodimer of 44 kDa. Members of the CD28 family share a number of common features. These receptors consist of paired V-set immunoglobulin superfamily (IgSF) domains attached to single transmembrane domains and cytoplasmic domains that contain critical signaling motifs. The CD28 and CTLA4 ligands, CD80 and CD86, consist of single V-set and C1-set IgSF domains. The interaction of these costimulatory receptors with ligands is mediated through the MYPPPY motif within the receptor V-set domains. CD28 is expressed constitutively on almost all human CD4 T cells and approximately 50% of CD8 T cells. CD28 costimulation has diverse effects on T cell function, including biochemical events at the immunological synapse, downstream phosphorylation and other post-translational modifications, transcriptional changes, and cytoskeletal remodeling. At the most basic level, CD28 signals increase a cell’s glycolytic rate, allowing cells to generate the energy necessary for growth and proliferation.
Why buy BioVision Products?
 |
 |
 |
 |
Innovation |
Affordability |
Global Presence |
Technical Support |
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways. |
BioVision is committed to providing the highest quality products at a competitive price. |
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery. |
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently. |
