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Human CellExp™ ADAM12, human recombinant

Involved in diverse processes such as asthma, development, angiogenesis and cancer
Catalog #: 7239
$190.00

Product Details

Cat # +Size 7239-10
Size 10 μg
Alternate Name ADAM12, MCMP, MLTN, MLTNA, MCMPMltna, Meltrin Alpha
Gene Symbol ADAM12
Gene ID 8038
Accession # AAH60804.1
Source HEK 293 cells
Appearance Lyophilized powder
Physical Form Description Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular Weight The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 55.2 kDa (Pro) and 34 kDa (Mature). The predicted N-terminus is Arg 29. DTT-reduced Protein migrates as 23 kDa, 46 kDa and 65 kDa due to glycosylation, corresponding to the prop
Purity by SDS-PAGE ≥95%
Endotoxin Level < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity Measured by its ability to cleave IGFBP-3. The bioactivity was measured in 100 μL reaction mixture containing 20 µg/ml of rhADAM12, 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5.
Reconstitution Instructions Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

A Disintegrin And Metalloprotease 12 (ADAM12) is also known as Meltrin-alpha and MCMP, is a member of the ADAM and MDC (Metalloprotease, Disintegrin, Cysteine-rich) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. Over 30 members have been identified and about half of them are active metalloproteases such as ADAM8, 9, 10, 12 and 17/TACE. ADAM12 has four Isoform, Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. ADAM12 Involved in skeletal muscle regeneration, specifically at the onset of cell fusion and also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.


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