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Product Details
| Cat # +Size | K244-100 |
|---|---|
| Size | 100 assays |
| Detection Method | Fluorescence (Ex/Em= 400/505 nm). |
| Species Reactivity | Mammalian |
| Applications | The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Calpain 1 inhibitors. Inhibitor control (Calpain inhibitor 1, ALLN) is included to compare the efficacy of test inhibitors. |
| Features & Benefits | • Simple procedure • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable |
| Kit Components | • Assay Buffer • Substrate • Calpain 1 Enzyme • Inhibitor Control (Calpain Inhibitor 1, ALLN) [1 μM] |
| Storage Conditions | -80°C |
| Shipping Conditions | Dry Ice |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Calpain 1 is calcium-dependent, non-lysosomal cysteine protease with low calcium requirement. It is expressed ubiquitously and in the brain, mainly localized to the cell body and dendrites of neurons and to a lesser extent in axons and glial cells. Hyperactivation of calpains is implicated in a number of pathologies associated with altered calcium homeostasis such as Alzheimer’s disease as well as secondary degeneration resulting from acute cellular stress following cerebral (neuronal) ischemia, traumatic brain injury and spinal cord injury. BioVision’s human calpain 1 inhibitor screening kit is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC possess very weak fluorescence; upon cleavage of the substrate by calpain 1, free AFC can be quantified fluorometrically (Ex/Em = 400/505 nm). In the presence of potent Calpain 1 inhibitor, the hydrolyzation of substrate will be impeded. The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Calpain 1 inhibitors. Inhibitor control (Calpain inhibitor 1, ALLN) is included to compare the efficacy of test inhibitors.
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BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways. |
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