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HSP90α Inhibitor Screening Kit (Fluorometric)

fOR Screening or characterizing HSP90α inhibitors
Catalog #: K2106
$545.00

Product Details

Cat # +Size K2106-100
Size 100 assays
Kit Summary BioVision’s HSP90α Inhibitor Screening Kit is a 96-well fluorometric assay that can be used to screen and characterize the potential inhibitors of HSP90α.
Detection Method Fluorescence (Ex/Em = 535/587 nm)
Applications For screening or characterizing HSP90α inhibitors
Features & Benefits The assay is simple, rapid and provides a reliable test for screening HSP90α inhibitors.
Kit Components ● HSP Assay Buffer
● LDH
● LDH Developer
● LDH Probe
● ATP
● HSP90α
● Geldanamycin
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

Heat shock protein (HSP) 90 is an ATP-dependent molecular chaperone that assists other proteins to fold properly, stabilizes proteins against heat stress, and assists in protein degradation. It has two isoforms (HSP90α and HSP90β) and both are abundantly expressed in the cytoplasm. Heat stress can cause proteins to denature thereby resulting in the loss of protein function. However, HSP90 can bind to the proteins during heat stress and protect them from misfolding and aggregation. Since HSP90 stabilizes various proteins including cancer-related proteins, cancer-associated protein kinases, transcription factors etc. that are required for the survival of cancer cells, HSP90 is considered as a potential target for cancer therapeutics. HSP90 inhibitors can target either the N-terminal ATP binding site or the C-terminal site of HSP90. Additionally, a number of HSP90 inhibitors are in clinical trials. BioVision’s HSP90α Inhibitor Screening Kit is a 96-well fluorometric assay that can be used to screen and characterize the potential inhibitors of HSP90α. The assay is based on measuring the HSP90-dependent refolding of thermally denatured Lactate Dehydrogenase (LDH) in the presence and absence of potential HSP90α inhibitors. In the presence of potential HSP90α inhibitors, refolding of thermally denatured LDH will be arrested resulting in reduced LDH activity, which is measured by fluorescence at Ex/Em = 535/587 nm. Geldanamycin is included in the kit as a positive control. The assay is simple, rapid and provides a reliable test for screening HSP90α inhibitors.


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